Short Communication Artificial duplicate reads in sequencing data of 454 Genome Sequencer FLX System
نویسندگان
چکیده
Hui Dong1, Yangyi Chen2, Yan Shen2, Shengyue Wang1, Guoping Zhao1,2*, and Weirong Jin1,2* Chinese National Human Genome Center at Shanghai, Shanghai 201203, China National Engineering Center for Biochip at Shanghai, Shanghai 201203, China *Correspondence address. Tel: þ86-21-51320288 (G.Z.)/þ86-21-51320298 (W.J.); Fax: þ86-21-51320288 (G.Z.)/þ86-21-51320298 (W.J.); E-mail: [email protected] (G.Z.)/[email protected] (W.J.)
منابع مشابه
Artificial duplicate reads in sequencing data of 454 Genome Sequencer FLX System.
The 454 Genome Sequencer (GS) FLX System is one of the next-generation sequencing systems featured by long reads, high accuracy, and ultra-high throughput. Based on the mechanism of emulsion PCR, a unique DNA template would only generate a unique sequence read after being amplified and sequenced on GS FLX. However, biased amplification of DNA templates might occur in the process of emulsion PCR...
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